Prior to Michigan
15. B. W. Biggs and K. E. J. Tyo. Aromatic natural products synthesis from aromatic lignin monomers using Acinetobacter baylyi ADP1. (bioRxiv)
14. B. W. Biggs, M. N. Price, D. Lai, J. Escobedo, Y. Fortanel, Y. Y. Huang, K. Kim, V. V. Trotter, J. V. Kuehl, L. M. Lui, R. Chakraborty, A. M. Deutschbauer, A. P. Arkin. High-throughput protein characterization by complementation using DNA barcoded fragment libraries. Molecular Systems Biology. 2024. (PMID: PMC11535334)
13. B. W. Biggs*, A. M. de Paz*, N. J. Bhan, T. R. Cybulski, G. M. Church, K. E.J. Tyo. Engineering Ca2+-dependent DNA polymerase activity. ACS Synthetic Biology. 2023. (PMID: 37856140)
12. B. W. Biggs, H. S. Alper, B. F. Pfleger, K. E.J. Tyo, C. N.S. Santos, P. K. Ajikumar, Gregory Stephanopoulos. Enabling commercial success of industrial biotechnology. Science. 2022. (PMID: 34941395)
11. E. Arvay, B. W. Biggs, L. Guerrero, V. Jiang, K. Tyo. Engineering Acinetobacter baylyi ADP1 for mevalonate production from lignin monomers. Metabolic Engineering Communications. 2021. (PMID: PMC8367835)
10. C. J. Glasscock, B. W. Biggs, J. T. Lazar, J. H. Arnold, L. A. Burdette, A. Valdes, M. K. Kang, D. Tullman-Ercek, K. E.J. Tyo, and J. B. Lucks. Dynamic control of pathway expression with riboregulated switchable feedback promoters. ACS Synthetic Biology. 2021. (PMID: PMC8141045)
9. B. W. Biggs, S. Bedore, E. Arvay, S. Huang, H. Subramanian, E. A. McIntyre, C. V. Duscent-Maitland, E. L. Neidle, and K. E.J. Tyo. Development of a genetic toolset for the highly engineerable and metabolically versatile Acinetobacter baylyi ADP1. Nucleic Acids Research. 2020. (PMID: PMC7229861)
8. A. M. Love*, Bradley W. Biggs*, K. E.J. Tyo, P. K. Ajikumar. Chemically Inducible Chromosomal Evolution (CIChE) for multi-copy metabolic pathway engineering in Escherichia coli. Methods in Molecular Biology: Microbial Metabolic Engineering. 2019. (PMID: PMC7055959)
7. T. Vogl, T. Kickenweiz, J. Pitzer, L. Sturmberger, A. Weninger, B. W. Biggs, E. Köhler, A. Baumschlager, J. E. Fischer, P. Hyden, M. Wagner, M. Baumann, N. Borth, M. Geier, P. K. Ajikumar, A. Glieder. Engineered bidirectional promoters enable rapid multi-gene co-expression optimization. Nature Communications. 2018. (PMID: PMC6123417)
6. D. A. Pertusi, M. E. Moura, J. G. Jeffreys, S. Prabhu, B. W. Biggs, K. EJ Tyo. Predicting novel substrates for enzymes with minimal experimental effort with active learning. Metabolic Engineering. 2017. (PMID: PMC7055960)
5. J. E. Rouck, B. W. Biggs, A. Kambalyal, W. R. Arnold, M. De Mey, P. K. Ajikumar, A. Das. Heterologous Expression and Characterization of Plant Taxadiene-5α-Hydroxylase (CYP725A4) in Escherichia coli. Protein Expression and Purification. 2017. (PMID: PMC5741078)
4. B. W. Biggs, J. E. Rouck, A. Kambalyal, C. G. Lim, M. De Mey, C. Starks, M. O’Neil, A. Das, P. K. Ajikumar. Orthogonal Assays Clarify the Oxidative Biochemistry of Taxol P450 CYP725A4. ACS Chemical Biology. 2016. (PMID: 26930136)
3. B. W. Biggs, C. G. Lim, K. Sagliani, S. Shankar, G. Stephanopoulos, M. De Mey, P. K. Ajikumar. Overcoming Heterologous Protein Interdependency to Optimize P450-Mediated Taxol Precursor Synthesis in Escherichia coli. Proceedings of the National Academy of Sciences. 2016. (PMID: PMC4812725)
2. B. W. Biggs, B. De Paepe, C. N. S. Santos, M. De Mey, and P. K. Ajikumar. Multivariate modular metabolic engineering for pathway and strain optimization. Current Opinion in Biotechnology. 2014. (PMID: 24927371)
1. B. W. Biggs, H. K. Hunt, A. M. Armani. Selective Patterning of Si-based Biosensor Surfaces Using Isotropic Silicon Etchants. Journal of Colloid and Interface Science 369. 2012. (PMID: PMC3265681)